Journal: Scientific Reports

Article Title: Human immune cells infiltrate the spinal cord and impair recovery after spinal cord injury in humanized mice

doi: 10.1038/s41598-019-55729-z

Figure Lengend Snippet: Human innate and adaptive immune cells from hNSG mice are functional and respond to cell-specific stimulation. ( A ) Human splenocytes upregulate cell surface expression of activation marker CD69 48 hours after stimulation with human CD3/28 antibody and rhIL2. ( B ) Proportion of hCD4 + and hCD8 + T cells expressing CD69 48 hours after stimulation by hCD3/28 and rhIL2. ( C ) Decrease in CFSE staining demonstrating robust proliferation of human splenocytes stimulated with hCD3/28 and rhIL2. ( D ) Proportion of proliferating splenocytes 96 hours after cell specific stimulation. ( E , F ) Quantification of human interferon gamma (IFNγ) and IL10 in culture supernatants after 96 hours of cell specific stimulation. ( G ) Human TNFα quantification in blood serum 1 hour after in vivo injection with 3 mg/kg lipopolysaccharide (LPS). Human IgG ( H ) and IgM ( I ) from blood serum in hNSG mice. Note the absence of human cytokines and antibodies in blood serum of non-engrafted NSG mice treated with LPS, demonstrating species specificity of ELISAs. ND = not detected. Data average ± SEM; n = 2 biological replicates in ( B , D ) n = 4 biological replicates in ( E , F ) n = 3 mice per group in ( G , H ) n = 3 NSG and n = 6 hNSG mice in ( I , J ).

Article Snippet: For T cell stimulation, ImmunoCult Human CD3/CD28 T cell Activator and 20 ng rhIL2 (Stemcell Technologies) was added to culture medium.

Techniques: Functional Assay, Expressing, Activation Assay, Marker, Staining, In Vivo, Injection